cag ires gfp pcag ires gfp empty a gift  (Addgene inc)

Journal: BMC Cancer

Article Title: Identification and modulation of a PI3K/AKT/mTOR pathway-targeting microRNA in order to increase colorectal cancer cells radiosensitivity in vitro

doi: 10.1186/s12885-025-14501-5

Figure Lengend Snippet: Variations in LoVo Cell Line Gene Expression and miR-16-5p. Examination of gene expression and miR-16-5p levels in LoVo cell lines. Control, hsa-miR-16-5p transfection (miR-16-5p), pLenti-III transfection (plenti-III), 4 Gy irradiation (4 Gy), miR-16-5p transfection with 4 Gy irradiation (miR + 4 Gy), and pLenti-III transfection with 4 Gy irradiation (plenti + 4 Gy) are the groups that were examined. Three biological and three technical replicates of the following genes are displayed: a CCND1, b CCNE1, c MYC, d CDK4, e PIK3CA, f HSP90AB1, and g miR-16-5p. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: One day after seeding, cells were transfected with pLenti-III-GFP-hsa-miR-16-5p (hsa-miR-16-5p) and pLenti-III-GFP (plenti-III) as a negative control (NC) using polyfectamine transfection reagent (QIAGEN).

Techniques: Gene Expression, Control, Transfection, Irradiation

Journal: BMC Cancer

Article Title: Identification and modulation of a PI3K/AKT/mTOR pathway-targeting microRNA in order to increase colorectal cancer cells radiosensitivity in vitro

doi: 10.1186/s12885-025-14501-5

Figure Lengend Snippet: Variations in HT-29 Cell Line Gene Expression and hsa-miR-16-5p: Examination of gene expression and hsa-miR-16-5p levels in HT-29 cell lines. Control, hsa-miR-16-5p transfection (miR-16-5p), pLenti-III transfection (plenti-III), 4 Gy irradiation (4 Gy), miR-16-5p transfection with 4 Gy irradiation (miR + 4 Gy), and pLenti-III transfection with 4 Gy irradiation (plenti + 4 Gy) are the groups that were examined. Three biological and three technical replicates of the following genes are displayed: a CCND1, b CCNE1, c MYC, d CDK4, e PIK3CA, f HSP90AB1, and g miR-16-5p. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: One day after seeding, cells were transfected with pLenti-III-GFP-hsa-miR-16-5p (hsa-miR-16-5p) and pLenti-III-GFP (plenti-III) as a negative control (NC) using polyfectamine transfection reagent (QIAGEN).

Techniques: Gene Expression, Control, Transfection, Irradiation

Journal: BMC Cancer

Article Title: Identification and modulation of a PI3K/AKT/mTOR pathway-targeting microRNA in order to increase colorectal cancer cells radiosensitivity in vitro

doi: 10.1186/s12885-025-14501-5

Figure Lengend Snippet: hsa-miR-16-5p ability to inhibit proliferation and colony formation in CRC: Images of one of three replicates of colony formation in control, miR-16-5p transfection, and plenti-III transfection groups at different radiation doses are shown for ( a ) LoVo and ( b ) HT-29 cell lines. Survival curves using a linear-quadratic model are shown to assess the changes in survival fraction at different doses for the same groups in ( b ) LoVo and ( d ) HT-29 cell lines

Article Snippet: One day after seeding, cells were transfected with pLenti-III-GFP-hsa-miR-16-5p (hsa-miR-16-5p) and pLenti-III-GFP (plenti-III) as a negative control (NC) using polyfectamine transfection reagent (QIAGEN).

Techniques: Control, Transfection

Journal: BMC Cancer

Article Title: Identification and modulation of a PI3K/AKT/mTOR pathway-targeting microRNA in order to increase colorectal cancer cells radiosensitivity in vitro

doi: 10.1186/s12885-025-14501-5

Figure Lengend Snippet: The impact of hsa-miR-16-5p upregulation on programmed apoptotic cell death was assessed by flow cytometric analysis, as shown in the dot plots for the ( a ) LoVo and ( b ) HT-29 cell lines. In these graphs, the x-axis represents fluorescence data from the FL1 channel corresponding to Annexin V-FITC, while the y-axis indicates the presence of PI dye. The quadrants are Q1, Q2, Q3 and Q4 and categorize the cells into necrotic, late apoptotic, early apoptotic and viable populations. In addition, the bar graphs visually represent ( c ) the mean percentage of apoptotic, ( d ) necrotic and ( f ) viable cells for the LoVo cell line. ( e ) The mean percentage of apoptotic, ( g ) necrotic and ( h ) viable cells for the HT-29 cell line. The experimental groups analyzed included a control group (0 Gy), miR-16-5p transfection (0 Gy), pLenti-III transfection (4 Gy), control with 4 Gy irradiation, miR-16-5p transfection combined with 4 Gy irradiation, and pLenti-III transfection with 4 Gy irradiation. Statistical significance is denoted as non-significant (ns), with different levels of relevance indicated by * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001

Article Snippet: One day after seeding, cells were transfected with pLenti-III-GFP-hsa-miR-16-5p (hsa-miR-16-5p) and pLenti-III-GFP (plenti-III) as a negative control (NC) using polyfectamine transfection reagent (QIAGEN).

Techniques: Fluorescence, Control, Transfection, Irradiation